Three Sisters Springs Visitor Center manages land access to Three Sisters Springs, a protected natural area in Crystal River National Wildlife Refuge famous for manatee encounters and clear freshwater springs, accessible primarily by water for in-water recreation like snorkeling and kayaking during manatee season.

Paddles Outdoor Rentals is a family-owned and operated business in Crystal River, FL, offering kayak rentals, paddleboard rentals, water bike rentals, bike rentals, guided kayak tours, swim with manatee tours, party paddle board rentals, and eco-tours including sunset tours and visits to Three Sisters Springs. They are the closest rental facility to Three Sisters Springs with a state-of-the-art kayak launch system and prioritize customer service.

The Plantation Adventure Center at Plantation Resort on Crystal River offers fishing charters, boat rentals, manatee swimming tours, snorkeling, scalloping, boating, kayaking, paddleboarding, and scenic cruises, with direct marina access to Kings Bay, Crystal River, and the Gulf of Mexico. Experienced captains guide fishing trips and other adventures on Florida's Nature Coast.

River Ventures is the #1 tour operator in Crystal River and Homosassa, Florida, specializing in eco-tours including manatee swims and snorkeling, airboat rides, scallop hunts, wildlife encounters, river cruises, paddling, kayak rentals, and lodging. They emphasize integrity, reputation, community outreach, education, high-quality facilities and vessels, flexibility, environmental dedication, and customer appreciation with a passionate crew.

Clear Kayaking Tours is a family-owned and operated business in Crystal River, Florida, with over 20 years of local experience, offering guided clear kayak tours through scenic waterways like Three Sisters Springs, focusing on unique underwater views of manatees and natural beauty.

Fun 2 Dive Manatee Tours is the #1 rated manatee tour company in Crystal River, Florida, operating since 1995. They specialize in small-group, semi-private, and private snorkel tours to swim with manatees in the Crystal River National Wildlife Refuge, with all necessary gear provided, USFWS permitted operations, and a maximum of 6-12 participants per boat.

A Crystal River Kayak Company offers kayak rentals, stand-up paddleboard (SUP), canoe rentals, and guided kayak tours including clear kayak adventures, manatee swim tours (November 15 - March 31), sunset tours, and dry tours to explore Crystal River's waterways, Three Sisters Springs, and Kings Bay for wildlife viewing like manatees. They provide the largest selection of boats (single/double sit-on-top, sit-inside, paddle boards, canoes, clear kayaks), closest dock to Three Sisters Springs (10-minute paddle), life jackets, paddles, rentals for masks/snorkels, wetsuits, accessories, gifts, apparel, changing rooms, restrooms, and water cooler. Reservations required for rentals and self-launches; welcomes all experience levels.

See Crystal River offers relaxing riverboat safaris and narrated sightseeing cruises in Crystal River National Wildlife Refuge, focusing on up-close wildlife encounters with manatees, alligators, turtles, and birds in their natural habitat. Expert USCG licensed captains provide narration about the area's ecology and history, using USCG inspected tour boats with a maximum of 16 passengers for an intimate experience.

Manatee Tour and Dive is a charter business in Crystal River, Florida, specializing in manatee tours, swimming with manatees in their natural habitat, scuba services, scalloping, and adventure expeditions. They provide expert guidance, personalized small group experiences (2 to 12 people, max 6 per vessel), and handle all details for stress-free travel with 24/7 support.

Crystal River Watersports is a family-owned adventure tour center operating for over 20 years in Crystal River, Florida, focusing on sustainable, eco-responsible opportunities to observe and preserve nature above, in, and under water. They offer in-water adventures including snorkeling, manatee swims, scuba tours and courses, scalloping, and mermaiding, as well as dry cruises, boat rentals with captains, ecology courses, and more, while promoting conservation through memberships in Save the Manatee Club Guardian Guides Program and other efforts.

Get Up and Go Kayaking is the original clear kayak tour company in Crystal River, established in 2018, offering small-group guided tours in 100% clear tandem kayaks through the spring-fed waters of Kings Bay and Three Sisters Springs. They provide two seasonal adventures: Manatee Season Tours (Nov 15–Mar 31) for manatee sightings, spring swimming, and wildlife viewing, and Three Sisters Springs Tours (Apr 1–Nov 14) for exploring springs like Jurassic, Magnolia, Hunter, and Three Sisters with opportunities for swimming and wildlife spotting. Tours last 2-2.5 hours, launch from Kings Bay Park, include expert guides, safety equipment, dry bags, and are limited to small groups of max 10 people for an exclusive experience.

Paddletail Waterfront Adventures is Crystal River's most professional tour company operating in Kings Bay, offering exclusive 'Swim with Manatees' tours for a once-in-a-lifetime encounter with majestic creatures in their natural habitat. They provide in-depth educational courses on passive observation, licensed and insured diving instructors on every tour, and options starting at $75 per person including VIP private tours for up to 6 guests on a custom heated houseboat with onboard restroom and changing room.